The Hebrew University of Jerusalem (HUJI; Hebrew: הַאוּנִיבֶרְסִיטָה הַעִבְרִית בִּירוּשָׁלַיִם) is a public research university based in Jerusalem, Israel. Co-founded by Albert Einstein and Dr. Chaim Weizmann in July 1918, the public university officially opened in April 1925. It is the second-oldest Israeli university, having been founded 30 years before the establishment of the State of Israel but six years after the older Technion university. The HUJI has three campuses in Jerusalem and one in Rehovot. The world's largest library for Jewish studies—the National Library of Israel—is located on its Edmond J. Safra campus in the Givat Ram neighbourhood of Jerusalem.

The university has five affiliated teaching hospitals (including the Hadassah Medical Center), seven faculties, more than 100 research centers, and 315 academic departments. As of 2018, one-third of all the doctoral candidates in Israel were studying at the HUJI.

Among its first few board of governors were Albert Einstein, Sigmund Freud, Martin Buber, and Chaim Weizmann. Four of Israel's prime ministers are alumni of the university. As of 2018, 15 Nobel Prize winners (8 alumni and teachers), two Fields Medalists (one alumnus), and three Turing Award winners have been affiliated with the HUJI.